FastQCFastQC Report
Fri 12 Jan 2018
HBR_Rep1_Aligned.sortedByCoord.out.bam

Summary

[OK]Basic Statistics

MeasureValue
FilenameHBR_Rep1_Aligned.sortedByCoord.out.bam
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1218
Sequences flagged as poor quality0
Sequence length36-100
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAACTCCGTCTCGTTGCAGCAGTATTTGAAGACCGTGTTGTTATGGT30.24630541871921183No Hit
CTTCGATCAGAAGGTCCACAATGTGTCCTTCGCCTTTGAGCTGATGCTGG30.24630541871921183No Hit
GTAGGTTTCTGTGGTTTAAGCCATTCAGTCTGTGGTCACATGTTACAGTA20.16420361247947454No Hit
CTCCCTTCTTGCCTCTCCAGACGTGTTCCCCGAGGAGATCTTCCTTCCGT20.16420361247947454No Hit
CCCAGCTAGAAAGCTAGCACAGTGAGCAGGGACCTGCCTTGCTCACTGTC20.16420361247947454No Hit
CTCATCAATAGGAACTGGGCAACCAGGTGGGGAGCCGCCAGGGGAGCTTG20.16420361247947454No Hit
CGGGGTTCCTGATGCAGATGGACAGATTCATGATCTTCCAGGCCTCCCGC20.16420361247947454No Hit
CGACAACCTCCTGACAACCTTTAGTGAACAGCTGCTCCCTGAGAAAGGGC20.16420361247947454No Hit
CACAGCTCCCCCGTCACTCCACGTTCTTGTACTTGGTGAACAGGTTGTAA20.16420361247947454No Hit
CGGCCTTCTCCGCCCGCAGGGTGGAAGCCTCCCGCCTCAGCGCCGAGTTG20.16420361247947454No Hit
CTCCAGCAACGCAGCCACTTCATGAAACTACAGGTGGTCAACTCTACAGG20.16420361247947454No Hit
CAGCAGGGCAATTGGGCTAACAGAGTTGATGGTGAGTGGGTGGCAGTAGT20.16420361247947454No Hit
CTCCTCATTGCTCATCACATACTTACCTGGGGACAATGAAATAGGTCTTT20.16420361247947454No Hit
TGCACATTTCCGGGTCTGTGAACCATACACAGACCACAAAGGCCGCTACC20.16420361247947454No Hit
CTATTTCGGACTGTGTCACCTCAGCCACATTCAGCTTGCACCCTGCCAGT20.16420361247947454No Hit
GTCCATCTAGTCAATGGGAGAGTACACAGCCACTCCAAAATAAAATATTT20.16420361247947454No Hit
CTTGCTTTCACTCAGTTCCTATGGACCAGGGTTCCTCTTCAAAGAACCAT20.16420361247947454No Hit
CTTTCTCCAATCGAGACCCCAAGCAGAGGTTCTCCCACTTTGCCAAGCCA20.16420361247947454No Hit
CGCGCTTCCCTCACCTATTCAGATACTTGCCGAGTGCCTGCCACTTGCCT20.16420361247947454No Hit
GTCTGTTCCACGTGGAAGGCCCTACACCCCAATCTTCTCCCCTACTCTGG20.16420361247947454No Hit
GTCCCAGGTCTCTGCCAGTGAGTCCAGCAGCTTCTGTCCCTCATAGAAGC20.16420361247947454No Hit
GTGGATGGCAGCCCACAGTTCTCAGAGACGCTGAGTGTGTGGAAGCTGGA20.16420361247947454No Hit
CTTCTCCGCCCGCAGGGTGGAAGCCTCCCGCCTCAGCGCCGAGTTGGTGA20.16420361247947454No Hit
CCGAAGCTTCCCTTGCCCCTGCTCCCTCCAGTAGTGGCCCCTTGGGGCTA20.16420361247947454No Hit
GTACTATTATCTCCATTTTATAGACAAGTCAACTGAGACTCACAGAGGTG20.16420361247947454No Hit
TGAACATCTTTCATGTGGAGCGATTGTATTTCCTCCTTTGTGGATTGTCA20.16420361247947454No Hit
AGACAGAAGAGAACTGAGCACAAAGAGAAAGTGATCTGTACACCTCCCAT20.16420361247947454No Hit
CTGGGACACTCCTGCCACCATCCACAGCTCCCCCGTCACTCCACGTTCTT20.16420361247947454No Hit
GGGCCTTCCACGTGGAACAGACAGCCCCTGTGTCTCTGTCTCTTGGGGAC20.16420361247947454No Hit
GGCCAGCCCTCTGCCCACCCGGGTAGCCCAAACCTGGCTCTGAGGGTGAT20.16420361247947454No Hit
CCGGGATGCCAGCAAGAGGGAGGCGAGCATGCAGTGAGCGATAGGTACAA20.16420361247947454No Hit
CGAGCACGGAATCTTTAAGAATCCGCCAGCACTTGGTAGCGTCTGCAGCC20.16420361247947454No Hit
GTCCTTCTTCACGGAGTACCTGCAGAACCAGCTGCTGACAAAAGGCATGG20.16420361247947454No Hit
GGCAGGAGTGCCATGCATCTCCGATAAGCCAGCTCTGTGGCCTTGGGCTG20.16420361247947454No Hit
ACCTGCCCTGTGGTCAGCCTGAGATGCAGCCCTTAGTATTACTATTGTTA20.16420361247947454No Hit
AGCTGAAAGCCCAGCCTTGGCAGCGTCTCCGAATGCCCAGATGGATGACG20.16420361247947454No Hit
AGCTGGGACACTCCTGCCACCATCCACAGCTCCCCCGTCACTCCACGTTC20.16420361247947454No Hit
TGAATGGCTGCTGGGACATGAGGCTTGGGTGTGAGATGCCCCGTCTGCTG20.16420361247947454No Hit
GTCCCAAGCCCACACACATAAGCACCAAGAGAAAAAACACAAATCACCAT20.16420361247947454No Hit
GGTTAATATCTTTAATATATACCGAGCTCATACAAATTTATCTGACCCCA20.16420361247947454No Hit
CCTGAATACAGGCTTTAGGTTCCCCTGGACTCCAGCCAAGGCTGTTCAGG20.16420361247947454No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)